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You are here:Home » Antibodies » Abs to Receptors, Cholinergic » Anti -Muscarinic Receptor M1 (Cholinergic Muscarinic Receptor M1, Chrm1)

Anti -Muscarinic Receptor M1 (Cholinergic Muscarinic Receptor M1, Chrm1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IH
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.
Catalog #M9520-03A
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:800 using whole rat brain homogenate.
Immunohistochemistry: 1:5000 using ABC techniques on PLP fixed rat brain sections
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot determined
FormSupplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 10mg/ml BSA.
PuritySerum
ImmunogenPeptide analog of the carboxyl terminal of the muscarinic M1 receptor covalently attached onto a carrier protein.
SpecificityRecognizes M1 Muscarinic Receptor (aa451-460). Crossreacts with full-length M1 Muscarinic Receptor, 60%. Does not react with M2 Muscarinic Receptor (aa457-466), M2 Muscarinic Receptor, M3 Muscarinic Receptor (aa580-589), M3 Muscarinic Receptor, M4 Muscarinic Receptor (aa469-478), M4 Muscarinic Receptor, M5 Muscarinic Receptor (aa519-531) or M5 Muscarinic Receptor.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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