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You are here:Home » Antibodies » Abs to Receptors, Cholinergic » Anti -Muscarinic Receptor M2, aa457-466 (Cholinergic Muscarinic Receptor M2, Chrm2)

Anti -Muscarinic Receptor M2, aa457-466 (Cholinergic Muscarinic Receptor M2, Chrm2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B IH
Catalog #M9520-13
ApplicationsSuitable for use in Western Blot, ELISA, and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 20ug/ml using whole rat brain homogenate
Immunohistochemistry: 5ug/ml stains PLP rat brain sections known to express the muscarinic M1 receptor using ABC techniques.
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies I1904-39 IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 40% glycerol.
PurityPurified by ammonium sulfate precipitation, dialysis and chromatography.
ImmunogenPeptide analogue of the carboxyl terminal of the M2 receptor covalently attached onto a carrier protein.
SpecificityShown to be specific for the COOH terminal of the M2 receptor. M2 Muscarinic Receptor (457-466) 100% M2 Muscarinic Receptor 50% M1 Muscarinic Receptor (451-460) 0% M3 Muscarinic Receptor (580-589 0% M3 Muscarinic Receptor 0% M4 Muscarinic Receptor (469-478) 0% M4 Muscarinic Receptor 0% M5 Muscarinic Receptor (519-531) 0% M5 Muscarinic Receptor 0%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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