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You are here:Home » Antibodies » Abs to Receptors, Cholinergic » Anti -Muscarinic Acetylcholine Receptor M2, aa225-359 (Chrm2, CHRM-2, 7TM Receptor, FLJ43243, HM2, MGC120006, MGC120007)

Anti -Muscarinic Acetylcholine Receptor M2, aa225-359 (Chrm2, CHRM-2, 7TM Receptor,
FLJ43243, HM2, MGC120006, MGC120007)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified IH IC
Catalog #M9520-13B
ApplicationsSuitable for use in Immunohistochemistry and Immunocytochemistry. Does not work on paraffin embedded tissues, and Immunoblotting. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (4% paraformaldehyde fixed tissue): 1-5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
IMMUNOHISTOCHEMISTRY PROTOCOL
Use on 30 um, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation.
1. 3 x 10 minute washes in TBS (with or without 0.25% Triton).
2. Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3. Incubate M9520-13B diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at RT followed by 16 hours at 4°C.
4. 3 x 10 minute washes in TBS.
5. Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).
6. 3 x 10 minute washes in TBS.
7. ABC Elite (1:200) in TBS.
8. 2 x 10 minute washes in TBS.
9. 1 x 10 minute wash in phosphate buffer (no saline).
10. DAB reaction with 0.06% NiCl added for intensification.
11. 2 x 10 minute washes in PBS.
12. 1 x 10 minute wash in phosphate buffer (no saline).
Clone TypeMonoclonal
IsotypeIgG2a
Clone No2Q147
HostRat
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified immunoglobulin
ImmunogenI3 loop of m2 receptor fusion protein (225-359), fused to Glutathione S-transferase.
SpecificityM2 muscarinic acetylcholine receptor. No reactivity with the other subtypes. Species crossreactivity: Human, monkey and rat. Expected to react with most mammalian species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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