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You are here:Home » Antibodies » Abs to Receptors, Cholinergic » Anti -Muscarinic Receptor M3, aa580-589 (Cholinergic Muscarinic Receptor M3, Chrm3)

Anti -Muscarinic Receptor M3, aa580-589 (Cholinergic Muscarinic Receptor M3, Chrm3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B IH
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and stimulates secretion in glandular tissue.
Catalog #M9520-21
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: 10ug/ml stains rat brain sections that express the muscarinic M3 receptor after fixing with PLP fixative and using ABC techniques.
Western Blot: 50ug/ml stains whole rat brain homogenates.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 10mg/ml BSA.
PurityPurified by precipitation, dialysis and chromatography.
ImmunogenA peptide analogue of the carboxyl terminal of the M3 receptor covalently attached to a carrier protein
SpecificityRecognizes M3 Muscarinic Receptor, aa580-589. Specific for the C-terminal of the M3 receptor. Crossreacts with M3 Muscarinic Receptor, 70%. Does not react with M1 Muscarinic Repeptor, M2 Muscarinic Receptor, M4 Muscarinic Receptor or M5 Muscarinic Receptor. This antibody is also specific for the COOH terminal of the M3 receptor as characterized by Western Blot, ELISA and tissue staining. Species Crossreactivity: human and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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