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You are here:Home » Antibodies » Abs to Receptors, Cholinergic » Anti -Muscarinic Receptor M3 (Cholinergic Muscarinic Receptor M3, Chrm3)

Anti -Muscarinic Receptor M3 (Cholinergic Muscarinic Receptor M3, Chrm3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IC
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and stimulates secretion in glandular tissue.
Catalog #M9520-21A
ApplicationsSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500
Immunocytochemistry: 1:2500
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, 10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot Determined
FormSupplied as a lyophilized powder. Reconstitute with 100ul sterile PBS containing 10mg/ml BSA.
PuritySerum
ImmunogenSynthetic peptide containing the C-terminal of the muscarinic M3 receptor (aa580-589) covalently attached onto a carrier protein.
SpecificityRecognizes the C-terminal of the M3 receptor. Recognizes M3 Muscarinic Receptor (580-589) (100%) and full-length M3 Muscarinic Receptor (70%). Does not react with M1 Muscarinic Receptor, M2 Muscarinic Receptor, M4 Muscarinic Receptor or the M5 Muscarinic Receptor. Species Crossreactivity: human and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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