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You are here:Home » Antibodies » Antibodies-Receptors, Cholinergic » Anti -Muscarinic Receptor M3 (Cholinergic Muscarinic Receptor M3, Chrm3)

Anti -Muscarinic Receptor M3 (Cholinergic Muscarinic Receptor M3, Chrm3)


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Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IH
Catalog #M9520-21D
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: Further dilute stock solution 1:8 with additional buffer (see WB protocol). This antibody should be sufficient for at least 20 lanes.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS containing 10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as a lyophilized powder. Reconstitute with 5.0 ml of sterile PBS containing10 mgm/ml BSA.
PurityPurified by precipitation, dialysis and chromatography.
ImmunogenPeptide analog of the carboxyl terminal of the M3 receptor covalently attached onto a carrier protein.
SpecificityRecognizes M3 Muscarinic Receptor (580-589) (100%) and full-length M3 Muscarinic Receptor (70%). Does not react with M1 Muscarinic Receptor (451-460), M1 Muscarinic Receptor, M2 Muscarinic Receptor (457-466), M2 Muscarinic Receptor, M4 Muscarinic Receptor (469-478), M4 Muscarinic Receptor, M5 Muscarinic Receptor (519-531) and M5 Muscarinic Receptor. Has been found to stain specifically the M3 receptor in Western immunoblots of whole rat brain homogenates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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