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You are here:Home » Antibodies » Abs to Receptors, Cholinergic » Anti -Muscarinic Receptor M3, aa580-589 (Cholinergic Muscarinic Receptor M3, Chrm3)

Anti -Muscarinic Receptor M3, aa580-589 (Cholinergic Muscarinic Receptor M3, Chrm3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IC
Catalog #M9520-22
ApplicationsSuitable for Immunocytochemical and Western Blot detection of the receptor.
Recommended DilutionImmunocytochemistry: 1:2500. This product has been found to stain PLP fixed rat brain sections known to express the muscarinic M3 receptor using ABC techniques.
Western Blot: 1:500 (Whole rat brain homogenate)
Optimal dilutions to be determined by the researcher.
Reconstitution0.1ml PBS containing 10mg/ml BSA.
Blocking PeptideRefer to catalog M9520-20.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume (100ul) by adding sterile 25% glycerol containing 10mg/ml BSA and store at -20°C. May be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized, neat serum. Reconstitute with 0.1ml PBS containing 10mg/ml BSA for the equivalent concentration in whole antiserum,or with additional buffer for more dilute antibody.
PuritySupplied as a sterile-filtered, defibrinated neat serum.
ImmunogenA peptide analogue of the carboxyl terminal of the Muscarinic M3 receptor covalently attached to a carrier protein..
SpecificityM3 Muscarinic Receptor (580-589) 100% M3 Muscarinic Receptor 70% M1 Muscarinic Receptor (451-460) 0% M1 Muscarinic Receptor 0% M2 Muscarinic Receptor (457-466) 0% M2 Muscarinic Receptor 0% M4 Muscarinic Receptor (469-478) 0% M4 Muscarinic Receptor 0% M5 Muscarinic Receptor (419-531) 0% M5 Muscarinic Receptor 0%
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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