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You are here:Home » Antibodies » Abs to Receptors, Cholinergic » Anti -Muscarinic Receptor M4 (Cholinergic Muscarinic Receptor M4, Chrm4)

Anti -Muscarinic Receptor M4 (Cholinergic Muscarinic Receptor M4, Chrm4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IC
Muscarinic acetylcholine receptor M4 has been reported to be expressed in many regions of the rat brain, particularly the striatum and hippocampus. ESTs have been isolated from human breast and eye libraries.
Catalog #M9520-31F
ApplicationsSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:8 diluted in 2% normal goat serum, TBS/Tween-20.
Optimal dilutions to be determined by the researcher.
Included Control PeptideM9520-31F1: Muscarinic Receptor M4 (460-478)
Reconstitute with 600ul of reconstituted antibody.
Peptide Amino Acid Sequence NH2-Lys-Lys-Thr-Phe-Arg-His-Leu-Leu-Leu-Ser-Gln-Tyr-Arg-Asn-Ile-Gly-Thr-Ala- Arg-COOH
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 10mg/ml BSA, PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot determined
FormSupplied as a lyophilized powder. Reconstitution with 5ml of PBS, 10mg/ml BSA.
PurityPurified by precipitation, dialysis and chromatography.
ImmunogenPeptide analog of the carboxyl terminal of the M4 receptor covalently attached onto a carrier protein.
SpecificityRecognizes the Muscarinic Receptor M4. Reactivity with the M4 Receptor (460-478) is 100% and 80% for the full-length M4 Receptor. Does not react with M1, M2, M3 or M5 Receptor. Specifically stains the M4 receptor in Western Blots of whole rat brain homogenates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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