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You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -MutS

Anti -MutS

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E
Catalog #M9557-01
E. coli-derived MutS is a protein component of the mismatch repair pathway, selectively binding DNA fragments containing mispaired or unpaired bases. MutS binds mismatches that most resemble base pairs (i.e., GT mismatches in G:C rich regions), allowing MutS to be used for mutation/SNP detection and isolation applications and mismatch repair structure/function studies.
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionsELISA: 1:1000-1:10,000; Recommended dilution buffer for use: 1X PBS, 3% BSA (IgG-free). The antibody is unlabeled, therefore detection may be accomplished with any appropriately labeled anti-rabbit secondary antibody. Alternatively, plates may be coated with a 1:1000 dilution of antibody in 0.05M Na2CO3, pH 9.6.
Optimal dilutions to be determined by the researcher.
Nuclease ActivityNo detectable nuclease activity when incubated with single or double stranded DNA for 16 hours at 37°C.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceE. coli
Concentration~0.3mg/ml
FormSupplied as a liquid in 78mM sodium chloride, 6mM K2HPO4, pH 7.4, 1.3mM potassium chloride, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenFull length E. coli MutS
SpecificityRecognizes E. coli MutS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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