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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E

Catalog #	M9557-01
E. coli-derived MutS is a protein component of the mismatch repair pathway, selectively binding DNA fragments containing mispaired or unpaired bases. MutS binds mismatches that most resemble base pairs (i.e., G	T mismatches in G:C rich regions), allowing MutS to be used for mutation/SNP detection and isolation applications and mismatch repair structure/function studies.
Nuclease Activity	No detectable nuclease activity when incubated with single or double stranded DNA for 16 hours at 37°C.
Proteinase Activity	No significant degradation of protein as judged by SDS-PAGE analysis, or loss of activity, after 16 hours at 37°C.
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions	ELISA: 1:1000-1:10,000; Recommended dilution buffer for use: 1X PBS, 3% (w/v) BSA (IgG-free BSA). The antibody is unlabeled, therefore detection may be accomplished with any appropriately labeled anti-rabbit secondary antibody. Alternatively, plates may be coated with a 1:1000 dilution of antibody in 0.05M Na2CO3 Buffer, pH 9.6.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	E. coli
Concentration	~0.3mg/ml
Form	Supplied as a liquid in 78mM sodium chloride, 6mM K2HPO4, pH 7.4, 1.3mM potassium chloride, 50% glycerol.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Full length E. coli MutS
Specificity	Recognizes E. coli MutS.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.