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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -Myb (c-myb, C-myb, c-myb_CDS, efg, Myb proto-oncogene protein, v-myb myeloblastosis viral oncogene homolog (avian))

Anti -Myb (c-myb, C-myb, c-myb_CDS, efg, Myb proto-oncogene protein, v-myb myeloblastosis
viral oncogene homolog (avian))

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Transcription factor Myb is expressed in hematopoietic cells and immature gastroepithelial cells.
Catalog #M9600-03B
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 2-4ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:12.5-1:25 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlJurkat and Ls174T cells. Testis.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No5G83
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant v-myb protein. Cellular Localization: Cytoplasmic and nuclear
SpecificityRecognizes human Myb at ~80kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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