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You are here:Home » Antibodies » Antibodies-Infectious Disease Mycobacterium » Anti -Mycobacterium tuberculosis

Anti -Mycobacterium tuberculosis


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31kD, Ag85A, B and C. All three proteins show fibronectin binding properties and act as mycolyltransferases involved in the final stages of mycobacterial cell wall assembly.
Catalog #M9699-59
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:200.
Optimal dilutions to be determined by the researcher.
Culture MediumDulbeccos modified Eagles medium with 10% fetal calf serum.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No6F152
FormSupplied as a liquid in PBS, pH 7.2, 15mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenBacterial press extract from Mycobacterium tuberculosis H37Rv.
SpecificityRecognizes Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein. No crossreactivity to antigens from Listeria monocytogenes or Escherichia coli. Epitope is lacking in PPD (culture filtrate, heat treated and precipitated with trichloroacetic acid). Reacts strongly in ELISA with M. tuberculosis cell culture filtrate or whole cells directly coated onto the microtiter wells (1). In Western blotting after SDS-PAGE reacts strongly with Ag85C and not as strongly with Ag85A and Ag85B.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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