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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31kD, Ag85A, B and C. All three proteins show fibronectin binding properties and act as mycolyltransferases involved in the final stages of mycobacterial cell wall assembly.
Catalog #	M9699-59
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:200.
	Optimal dilutions to be determined by the researcher.
Culture Medium	Dulbeccos modified Eagles medium with 10% fetal calf serum.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6F152
Host	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide, before the addition of glycerol to 40%.
Purity	Purified by Protein A/G affinity chromatography from culture supernatant.
Immunogen	Bacterial press extract from Mycobacterium tuberculosis H37Rv.
Specificity	Recognizes Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein. No crossreactivity to antigens from Listeria monocytogenes or Escherichia coli. Epitope is lacking in PPD (culture filtrate, heat treated and precipitated with trichloroacetic acid). Reacts strongly in ELISA with M. tuberculosis cell culture filtrate or whole cells directly coated onto the microtiter wells (1). In Western blotting after SDS-PAGE reacts strongly with Ag85C and not as strongly with Ag85A and Ag85B.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.