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Anti -Mycobacterium tuberculosis, Early Secreted Antigenic Target Protein (ESAT6)
Anti -Mycobacterium tuberculosis, Early Secreted Antigenic Target Protein (ESAT6)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B |
|
| The ESAT-6 antigen from Mycobacterium tuberculosis is a dominant target for cell-mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models (2,5). | | | Catalog # | M9699-72 | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 1:3160 | | Optimal dilutions. to be determined by the researcher. | | Hybridoma | X63-Ag8.653. Female CF1 x Balb/c mice immunized i.p. | | Culture Medium | RPMI 1640 with 10% fetal calf serum. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 6F153 | | Host | Mouse | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide before the addition of glycerol to 40%. | | | Purity | Purified by Protein A/G affinity chromatography from culture supernatant. | | Immunogen | PPD from Mycobacterium tuberculosis (Tuberculin PPD, RT23, Statens Serum Institut) (1). | | Specificity | Recognizes a 10kD band from short term culture of M. tuberculosis identified as the protein ESAT-6 (Rv3875) in Western Blot. Binds to an epitope consisting of the residues EQQWNFAGIEAAA at positions 3-15 (4). Species Crossreactivity: Mycobacterium kansasii, Mycobacterium szulgai, and Mycobacterium marinum. Reacts with PPD, culture filtrate, cytosol and cell wall preparations from M. tuberculosis. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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