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You are here:Home » Antibodies » Abs to Infectious Disease Mycobacterium » Anti -Mycobacterium tuberculosis, Early Secreted Antigenic Target Protein (ESAT6)

Anti -Mycobacterium tuberculosis, Early Secreted Antigenic Target Protein (ESAT6)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
The ESAT-6 antigen from Mycobacterium tuberculosis is a dominant target for cell-mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models (2,5).
Catalog #M9699-72
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:3160
Optimal dilutions. to be determined by the researcher.
HybridomaX63-Ag8.653. Female CF1 x Balb/c mice immunized i.p.
Culture MediumRPMI 1640 with 10% fetal calf serum.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F153
HostMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 15mM sodium azide before the addition of glycerol to 40%.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenPPD from Mycobacterium tuberculosis (Tuberculin PPD, RT23, Statens Serum Institut) (1).
SpecificityRecognizes a 10kD band from short term culture of M. tuberculosis identified as the protein ESAT-6 (Rv3875) in Western Blot. Binds to an epitope consisting of the residues EQQWNFAGIEAAA at positions 3-15 (4). Species Crossreactivity: Mycobacterium kansasii, Mycobacterium szulgai, and Mycobacterium marinum. Reacts with PPD, culture filtrate, cytosol and cell wall preparations from M. tuberculosis.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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