| Human myelin basic protein (MBP) is a 20.115 Kd protein (186aa) found in myelin of the CNS and PNS. Expressed as an intracellular protein by oligodendrocytes and Schwann cells, MBP aids in the compaction and stability of myelin. |
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| Catalog # | M9758-01Q |
| Applications | Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested. |
| Recommended Dilutions | |
| Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissues |
| Immunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cells |
| Western Blot: 1:2000-1:5000 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1906 IgY, Chicken Pab Rb x Ch |
| I1906-10 IgY, Chicken (HRP) Rb x Ch |
| I1906-12 IgY, Chicken (FITC) Rb x Ch |
| I1906-14 IgY, Chicken (AP) Rb x Ch |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgY |
| Host | Chicken |
| Source | Mouse |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to Myelin Basic Protein (KLH). Species sequence homology: human (NP_002376, NCBI) and mouse (NP_034907, NCBI). |
| Specificity | Recognizes Myelin Basic Protein. Species crossreactivity |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
