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You are here:Home » Antibodies » Abs to Myelin » Anti -Myelin Basic Protein, phosphorylated (Thr98) (MBP)

Anti -Myelin Basic Protein, phosphorylated (Thr98) (MBP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
In vitro, myelin basic protein (MBP) is a substrate for phosphorylation by several different protein kinases including MAPK, PKA, calmodulin-dependent protein kinase, PKC and phosphorylase kinase. Even highly specific protein kinases such as Raf1, MEK and MEKK can utilize MBP as an alternative substrate. Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles. MBP serves as a marker for oligodendrocytes and Schwann cells. MBP is also a marker for malignant schwannomas and appears to play a significant role in the etiology of multiple sclerosis and experimental autoimmune encephalomyelitis.
Catalog #M9758-02B
ApplicationsSuitable for use in Western Blot and Protein Kinase assay. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 0.125-0.5ug/ml of this lot detected phosphorylated MBP after incubation with MAP Kinase 2/Erk2, active. Note: Some preparations of MBP contain basal levels of phosphorylated MBP.
Protein Kinase Assay: Phosphorylated MBP was detected in a direct kinase assay using the Non- Radioactive MAP Kinase Assay Kit. An Immunoprecipitation kinase assay was performed with this lot of antibody using the Non-Radioactive MAP Kinase.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5H22
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide containing the seqeunce..PRpTPP…in which pT corresponds to phosphorylated threonine residue 98 of phosphorylated human MBP (myelin basic protein). The immunizing sequence is identical in porcine, mouse, rat and bovine.
SpecificityRecognizes in vitro phosphorylated human myelin basic protein, Mr ~18-23kD. Species Crossreactivity: Broad species crossreactivity expected based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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