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You are here:Home » Antibodies » Abs to Myelin » Anti -Myelin Basic Protein (MBP)

Anti -Myelin Basic Protein (MBP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles. MBP serves as a marker for oligodendrocytes and Schwann cells. MBP is also a marker for malignant schwannomas and appears to play a significant role in the etiology of multiple sclerosis and experimental autoimmune encephalomyelitis.
Catalog #M9758-02F
ApplicationsSuitable for use in Western Blot, MAP Kinase Assay and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 0.05-0.5ug/ml detects MBP in RIPA lysates from mouse and rat brain tissue preparations. Mouse brain cytosol homogenate was resolved by electrophoresis, transferred to PVDF and probed with M9758-02F. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
MAP Kinase and Indirect ELISA: 0-200ng of M2362-06 was used to phosphorylate well-bound MBP. Phosphorylation of MBP was detected using M9758-05. Total MBP was detected by M9758-02F.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3J12
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to the human myelin basic protein sequence containing amino acid Thr98 (KLH).
SpecificityRecognizes human MBP, Mr 18-20kD.
Species CrossreactivityMouse and rat.
Species Sequence HomologyBovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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