Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified B IH

Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles. MBP serves as a marker for oligodendrocytes and Schwann cells. MBP is also a marker for malignant schwannomas and appears to play a significant role in the etiology of multiple sclerosis and experimental autoimmune encephalomyelitis.
Catalog #	M9758-03
Applications	Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended Dilution
	Immunohistochemistry (formalin fixed and paraffin embedded): 1:100 by PAP or ABC. Pretreatment of tissue with proteolytic enzyme is not required.
	Western Blot: 1:1500 in TTBS, overnight at 4°C.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 0.05M Tris HCl, pH 7.6, 15mM sodium azide before the addition of glycerol to 40%.
Purity	Purified immunoglobulin fraction from ammonium sulfate fraction.
Immunogen	Human myelin basic protein from brain
Specificity	Suitable for labeling of oligodendrocytes and myelin of white matter in brain and spinal cord (gray matter stains less abundantly). Also stains peripheral nerves.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.