USBio Logo

M9758-03D Rat Anti-Myelin Basic Protein, aa82-87 (MBP)

Specifications
References
Clone Type
Monoclonal
Host
Rat
Source
Bovine
Isotype
IgG
Clone Number
2Q292
Grade
Supernatant
Applications
E IHC RIA WB
Crossreactivity
Bo Hu Mo Rb Rt Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C

Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles (3). MBP serves as a marker for oligodendrocytes and Schwann cells (3). MBP is also a marker for malignant schwannomas (4) and appears to play a significant role in the etiology of multiple sclerosis (5-7) and experimental autoimmune encephalomyelitis (8).

Applications
Suitable for use in Western Blot, ELISA, RIA and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Bovine myelin basic protein.
Form
Supplied as a liquid, 0.09% sodium azide.
Purity
Supernatant
Specificity
Recognizes intact MBP and synthetic peptide aa82-99. Reacts weakly with peptides ending in the phe 91 where the 91-92 phe-phe bond is broken. Epitope: Mapped to the region DENPVV.|Species crossreactivity: Human, bovine, sheep, rabbit, mouse and rat.
References
1. Groome, N.P., et al., J. Neuroimmunol. (1986) 12:253-264. 2. Martenson, R.E. (1984) in Experimental Allergic Encephalomyelitis. A useful model for Multiple Sclerosis (Alvord, Kies and Suckling eds) pp 511-521. Alan Liss N.Y. 3. Glynn, P., et al., J. Neurochem (1987) 48:752-759. 4. Hruby, S., et al., Molecular Immunology In press. 4. Day, E.D. and Potter, N.T (1986) J. Neuroimmunol. 10, 289. 5. Hashim, G.A. (1978) Immunol. Rev. 39, 60. 6. Golo, M. (1987) Histochemistry 87, 201. 7. Mogollon, R., et al. (1984) Cancer 53, 1190. 7. Whitaker, J.N. (1984) Ann. N.Y. Acad. Sci. 436, 140. 9. Eidinoff, H. (1988) Med. Hypotheses 26, 103. 10. tar Meulen, V. (1988) Ann. N.Y. Acad. Sci. 540, 202. 11. Kerlero de Rosbo, N., et al. (1985) J. Neuroimmunol. 9, 349. 12. Sires, L.R., et al. (1981) Science 214, 87.
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved