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You are here:Home » Antibodies » Abs to Myelin » Anti -Myelin Basic Protein, phosphorylated (Thr98) (MBP) (HRP)

Anti -Myelin Basic Protein, phosphorylated (Thr98) (MBP) (HRP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B
In vitro, myelin basic protein (MBP) is a substrate for phosphorylation by several different protein kinases including MAPK, PKA, calmodulin-dependent protein kinase, PKC and phosphorylase kinase. Even highly specific protein kinases such as Raf1, MEK and MEKK can utilize MBP as an alternative substrate. Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles. MBP serves as a marker for oligodendrocytes and Schwann cells. MBP is also a marker for malignant schwannomas and appears to play a significant role in the etiology of multiple sclerosis and experimental autoimmune encephalomyelitis.
Catalog #M9758-05K
ApplicationsSuitable for use in ELISA and Immunoblot. Other applications not tested.
Recommended DilutionImmunoblot: 1:1000-1:5000. Dephosphorylated MBP was treated with MAP Kinase 2/Erk2, active for 20 minutes at 30°C. 1ug of the reaction mixture was loaded per lane. Resulting blots were probed with anti-phospho-MBP, HRP conjugate at 1:1000. Note: Some preparations of MBP contain basal levels of phosphorylated MBP, which are detected by the phospho-MBP antibody.
MAP Kinase Assay/ELISA 1:5000-1:10,000
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No0.T.90
HostMouse
SourceHuman
FormSupplied as a liquid in PBS, 30% glycerol.
PurityPurified
ImmunogenSynthetic peptide corresponding to the human myelin basic protein (MBP) sequence phosphorylated at Thr98 and coupled to tuberculin.
SpecificityMyelin Basic Protein, phosphorylated (MBP)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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