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You are here:Home » Antibodies » Abs to Myelin » Anti -Myelin Proteolipid Protein, CT (MPLP)

Anti -Myelin Proteolipid Protein, CT (MPLP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IC
This transmembrane proteolipid protein is the predominant myelin protein present in the central nervous system. It may play a role in the compaction, stabilization, and maintenance of myelin sheaths, as well as in oligodendrocyte development and axonal survival. Mutations in this gene cause X-linked Pelizaeus-Merzbacher disease and spastic paraplegia type 2. Alternatively spliced transcript variants encoding distinct isoforms or having different 5' UTRs, have been identified.
Catalog #M9758-14
ApplicationsSuitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: Can aggregate causing HMW bands to sometimes appear in certain preparations.
Immunocytochemistry: 4% PFA fixed cells for 5-10 minutes at RT. Use 0.1% Triton in block only to allow antibody access to C-terminal epitope.
Immunohistochemistry (Frozen): Fresh frozen sections or 4% PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2% Triton X-100 in the blocking step for permeabilization.
Immunohistochemistry (Paraffin): Works best with lightly fixed tissues, or Bouin's fixed material, although there are reports of traditional formalin fixation also working with antigen recovery.
Optimal dilutions to be determined by the researcher.
Positive ControlBrain
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H410
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to the C-terminal, CGRGTKF of the Myelin proteolipid protein.
SpecificityRecognizes the human myelin proteolipid protein (23-25kD). Crossreacts with DM20 (20kD). Species Crossreactivity: mammals
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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