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You are here:Home » Antibodies » Antibodies-Enzymes, Myeloperoxidase » Anti -Myeloperoxidase (MPO, pANCA)

Anti -Myeloperoxidase (MPO, pANCA)


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Clone Host Grade Applications
Polyclonal Human Purified E IF
In normal tissues and in a variety of myeloproliferative disorders, myeloid cells of both neutrophilic and eosinophilic types, at all stages of maturation, exhibit strong cytoplasmic reactivity for MPO. It is readily detected in myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythro-leukemia, myeloblastomas, and other hematopoietic disorders. Erythroid precursors, megakaryocytes, lymphoid cells, mast cells, and plasma cells are nonreactive.
Catalog #M9760
ApplicationsSuitable for use in ELISA and Immunofluorescence. Other applications not tested.
Recommended DilutionsImmunofluorescence: Exhibits a P-ANCA pattern
ELISA: 1:100
Optimal dilution determined by the researcher.
Autoantibody ActivityAnti-MPO Activity by ELISA: As reported (lot-specific). Current lot value: 96EU/ml
Interpretation of Results: <4EU/ml: Negative; 4.1-5.9EU/ml: Equivocal; >6EU/ml: Positive
Other Autoantibody Activity in the SerumThe serum exhibits no reactivity at a dilution of 1:100 when tested against purified SSA, SSB, SCL, Sm, Sm/RNP, Jo-1, mitochondrial or ribosomal antigens in an ELISA system designed to detect IgG antibody subclasses.
Antigen Result (EU/ml)
SSA, SCL, Sm 2
Sm/RNP, Jo-1 1
Interpretation of Results:
Negative: <16EU/ml, Equivocal: 16-20EU/ml, Positive: >20EU/ml
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationNot Determined
FormSupplied as a powder lyophilized from a solution containing PBS, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.
PurityFractionated from delipidated, defibrinated and precipitated human serum.
ImmunogenMyeloperoxidase (MPO). Autoantibody derived from donor human serum.
SpecificityRecognizes human Myeloperoxidase (MPO, pANCA)
Species CrossreactivityShould detect MPO from most species. Does not recognize PR-3, SS-A, SS-B, Sm, Sm/RNP, SCL and Jo-1 antigens at a 1:1000 dilution in ELISA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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