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You are here:Home » Antibodies » Abs to Enzymes, Myeloperoxidase » Anti -Myeloperoxidase (Glycerol Free) (MPO, pANCA)

Anti -Myeloperoxidase (Glycerol Free) (MPO, pANCA)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B
In normal tissues and in a variety of myeloproliferative disorders, myeloid cells of both neutrophilic and eosinophilic types, at all stages of maturation, exhibit strong cytoplasmic reactivity for MPO. It is readily detected in myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythro-leukemia, myeloblastomas, and other hematopoietic disorders. Erythroid precursors, megakaryocytes, lymphoid cells, mast cells, and plasma cells are nonreactive. Cells of monocytic derivation reveal variable reactivity and are typically weakly positive or nonreactive. Cells comprising the infiltrate of a spectrum of lymphoid malignancies, e.g., lymphoblastic lymphoma or leukemia, chronic lymphocytic leukemia, hairy cell leukemia, non-Hodgkin’s lymphomas of T-or B-cell type, and Hodgkin’s disease, are nonreactive, as are the non-neoplastic tissues present in these specimens, except for occasional cells of myeloid derivation. MPO is not observed in the neoplastic cells of a wide variety of epithelial tumors and sarcomas, or in the contiguous non-neoplastic tissues. MPO is useful in differentiating between myeloid and lymphoid leukemias.
Catalog #M9760-08A
ApplicationsSuitable for use in Western Blot and ELISA. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:4000; Strong reaction to 0.3 and 0.6ug antigen.
Optimal dilutions to be determined by the researcher.
TiterIndirect ELISA: 1:4000
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
PurityPurified by ammonium sulfate precipitation and DEAE ion exchange chromatography.
ImmunogenNeutrophil Myeloperoxidase
SpecificityRecognizes human neutrophil Myeloperoxidase. Single arc upon immunoelectrophoresis of human neutrophil granule extract. Single arc upon immunoelectrophoresis of antigen.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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