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You are here:Home » Antibodies » Abs to Enzymes, Myeloperoxidase » Anti -Myeloperoxidase (MPO, pANCA)

Anti -Myeloperoxidase (MPO, pANCA)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
In normal tissues and in a variety of myeloproliferative disorders, myeloid cells of both neutrophilic and eosinophilic types, at all stages of maturation, exhibit strong cytoplasmic reactivity for MPO. It is readily detected in myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythro-leukemia, myeloblastomas, and other hematopoietic disorders. Erythroid precursors, megakaryocytes, lymphoid cells, mast cells, and plasma cells are nonreactive. Cells of monocytic derivation reveal variable reactivity and are typically weakly positive or nonreactive. Cells comprising the infiltrate of a spectrum of lymphoid malignancies, e.g., lymphoblastic lymphoma or leukemia, chronic lymphocytic leukemia, hairy cell leukemia, non-Hodgkin’s lymphomas of T-or B-cell type, and Hodgkin’s disease, are nonreactive, as are the non-neoplastic tissues present in these specimens, except for occasional cells of myeloid derivation. MPO is not observed in the neoplastic cells of a wide variety of epithelial tumors and sarcomas, or in the contiguous non-neoplastic tissues. MPO is useful in differentiating between myeloid and lymphoid leukemias.
Catalog #M9760-10
ApplicationsSuitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
HybridomaHybridization of Sp 2/0 myeloma cells with spleen cells from Balb/c mice.
Recommended PairSuitable for use as the capture or solid phase antibody.
Matched to M9760-12 as the detection or conjugate (label) antibody.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No1.BB.82
HostMouse
SourceHuman
Concentration~5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity> 90%. Purified by chromatography on Protein A Sepharose. Purity is tested by electrophoresis.
ImmunogenHuman myeloperoxidase purified leukocyte mass
SpecificityReacts with human Myeloperoxidase.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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