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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IP IH

Myoglobin, an intracellular haemoprotein expressed in the heart and oxidative skeletal myofibres of vertebrates, binds molecular oxygen and may facilitate oxygen transport from erythrocytes to mitochondria, thereby maintaining cellular respiration during periods of high physiological demand (1). Myoglobin may serve a variety of functions in muscular oxygen supply, such as oxygen storage, facilitated O(2) diffusion, and myoglobin-mediated oxidative phosphorylation. Disruption of myoglobin results in the activation of multiple compensatory mechanisms that steepen the pO(2) gradient and reduce the diffusion path length for O(2) between capillary and the mitochondria (2).
Catalog #	M9800-27
Applications	Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000-1:5000
	Immunoprecipitation: 1:40
	Immunohistochemistry: 1:250-1:500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9E390
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Purity	Supernatant
Immunogen	Synthetic peptide corresponding to residues on the C-terminus of human myglobin.
Specificity	Recognizes human Myoglobin at ~17kD. Species crossreactivity: Mouse, rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.