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You are here:Home » Molecular Biology » MB-Serum Proteins » Myoglobin, Recombinant, Human (MB)

Myoglobin, Recombinant, Human (MB)

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Specifications

Myoglobin is a cytoplasmic hemoprotein, expressed solely in cardiac myocytes and oxidative skeletal muscle fibers, that reversibly binds O2 by its heme (iron-containing porphyrin) prosthetic group in the center around which the remaining apoprotein folds. The red color that meat takes is partly determined by the charge of the iron atom in myoglobin and the oxygen attached to it. When meat is in its raw state, the iron atom has a charge of +2 and is bound to an oxygen molecule. Myoglobin is a single-chain globular protein of 153aa and has a molecular weight of 16,700D. Unlike the blood-borne hemoglobin, to which it is structurally related, this protein does not exhibit cooperative binding of oxygen, since positive cooperaivity is a property of multimeric/oligomeric proteins only. Recent gene disruption studies using the myoglobin deficient mouse model support the conclusion that the functions of myoglobin include: facilitated oxygen transport, the storage of oxygen and a scavenger of nitric oxide or reactive oxygen species. Myoglobin is an early indicator of cardiac ischemia that achieves maximal sensitivity 3 to 4 hours after symptom onset. However, it is only marginally more sensitive than CK-MB early after the onset of symptoms, and it is less sensitive 6 to 8 hours after symptom onset. Because of rapid clearance from the blood, myoglobin may "miss" late-presenting patients, and it is less cardiospecific than CK-MB.
Catalog #M9800-35A
SourceRecombinant corresponding to 1-153aa from human Myoglobin, fused to N-His-tag expressed in E.coli.
Molecular Weight~19.5kD
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight~19.5kD
SourceE.coli
Purity~95% (SDS-PAGE)
ConcentrationNot determined
FormSupplied as a lyophilized powder from 20mM Tris, pH8.0, 10mM sodium chloride.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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