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You are here:Home » Molecular Biology » MB-Serum Proteins » Myoglobin, Recombinant, Human (MB)

Myoglobin, Recombinant, Human (MB)

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Specifications

Myoglobin is a cytoplasmic hemoprotein, expressed solely in cardiac myocytes and oxidative skeletal muscle fibers, that reversibly binds O2 by its heme residue, a porphyrin ring:iron ion complex. Since the initial discovery of its structure over 40 years ago, wide-ranging work by many investigators has added importantly to our understanding of its function and regulation. Functionally, myoglobin is well accepted as an O2-storage protein in muscle, capable of releasing O2 during periods of hypoxia or anoxia. Myoglobin is also thought to buffer intracellular O2 concentration when muscle activity increases and to facilitate intracellular O2 diffusion by providing a parallel path that augments simple diffusion of dissolved O2. The use of gene targeting and other molecular biological techniques has revealed important new insights into the developmental and environmental regulation of myoglobin and provided additional functions for this hemoprotein such as scavenging nitric oxide and reactive oxygen species.
Catalog #M9800-35B
Recombinant Myoglobin is assembled in E. coli as holo-form, which combines iron-porphyrin (heme) group during expressiion of the gene coding apoprotein in human heart muscle. Myoglobin (Mb) is a cytosolic protein comprising 153 amino acid residues with a molecular mass of 17.5kD.
ApplicationsSuitable for use as a standard for ELISA and Immunoblot, and as a highly qualified antigen for antibody production. Other applications not tested.
Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight17.5kD
SourceE. coli
Purity96% by SDS-PAGE
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, <0.05% sodium azide.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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