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You are here:Home » Molecular Biology » MB-Myosin » MYL5, Recombinant, Human, aa1-132, His-tag (Myosin Light Chain 5, Myosin Regulatory Light Chain 5, Superfast Myosin Regulatory Light Chain 2, MYLC2, MyLC-2)

MYL5, Recombinant, Human, aa1-132, His-tag (Myosin Light Chain 5, Myosin Regulatory Light
Chain 5, Superfast Myosin Regulatory Light Chain 2, MYLC2, MyLC-2)

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Specifications

Myosin regulatory light chain 5, also known as MYL5, is a hexameric ATPase cellular motor protein. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL5 is a regulatory light chain and is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia. Reconstitution of myosin with regulatory light chain 5 or alkali light chain increases filament velocity to intermediate rates, and readdition of both classes of light chains fully restores the original sliding velocity.
Catalog #M9848-04U
SourceRecombinant corresponding to aa1-132 from human MYL5, fused to His-tag at N-terminal expressed in E.coli.
AA SequenceMGSSHHHHHH SSGLVPRGSH MGSHMDQNRD GFIDKEDLKD TYASLGKTNV KDDELDAMLK EASGPINFTM FLNLFGEKLS GTDAEETILN AFKMLDPDGK GKINKEYIKR LLMSQADKMT AEEVDQMFQF ASIDVAGNLD YKALSYVITH GEEKEE
Molecular Weight~17.4kD (MALDI-TOF)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~17.4kD
SourceE.coli
Purity~90% (SDS-PAGE)
Concentration~0.5mg/ml
FormSupplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 1mM DTT, 30% glycerol, 0.1M sodium chloride.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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