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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin, Skeletal

Anti -Myosin, Skeletal

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IH
Myosin is the main constituent of the thick filaments of muscle fibres. It acts with actin in the contraction and relaxation of muscles.
Catalog #M9848-05A
ApplicationsSuitable for use in Immunohistochemistry. Reported to be suitable for Western Blot. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (paraffin): 1:10. Also suitable for use on frozen sections.
Optimal dilutions to be determined by the researcher.
Recommended Secondary ReagentsF(ab')2 rabbit anti-mouse IgG HRP
Recommended Positive Control TissueSkeletal muscle
Recommended Negative ControlsMouse IgG1 Negative Control
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2763
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, 1% BSA.
PurityPurified IgG.
ImmunogenMyosin isolated from rabbit skeletal muscle.
Specificity
Recognizes skeletal myosin, specifically the fast twitch (type II) isomyosin molecules of the heavy chain of human or animal myosin. Species CrossreactivityHuman, rabbit, mouse, rat, bovine, chicken, and guinea pig
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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