| Myosin is the main constituent of the thick filaments of muscle fibres. It acts with actin in the contraction and relaxation of muscles. |
|
| Catalog # | M9848-05A |
| Applications | Suitable for use in Immunohistochemistry. Reported to be suitable for Western Blot. Other applications have not been tested. |
| Recommended Dilution | Immunohistochemistry (paraffin): 1:10. Also suitable for use on frozen sections. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Reagents | F(ab')2 rabbit anti-mouse IgG HRP |
| Recommended Positive Control Tissue | Skeletal muscle |
| Recommended Negative Controls | Mouse IgG1 Negative Control |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H2763 |
| Host | Mouse |
| Source | Human |
| Concentration | As reported |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, 1% BSA. |
| Purity | Purified IgG. |
| Immunogen | Myosin isolated from rabbit skeletal muscle. |
| Specificity | |
| Recognizes skeletal myosin, specifically the fast twitch (type II) isomyosin molecules of the heavy chain of human or animal myosin. Species Crossreactivity | Human, rabbit, mouse, rat, bovine, chicken, and guinea pig |
|
|
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
