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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin IIA, Non-muscle (Cellular Myosin Heavy Chain Type A, DFNA17, EPSTS, FTNS, MGC104539, MHA, MYH2A, MYH9, MYHas8, MyHC-2A, MyHC-IIa, MYHSA2, Myosin 9, Myosin Heavy Chain 9, Myosin Heavy Chain 9 Non-muscle, Myosin Heavy Chain Non-muscle IIa, Myosin Hea

Anti -Myosin IIA, Non-muscle (Cellular Myosin Heavy Chain Type A, DFNA17, EPSTS, FTNS,
MGC104539, MHA, MYH2A, MYH9, MYHas8, MyHC-2A, MyHC-IIa, MYHSA2, Myosin 9, Myosin Heavy
Chain 9, Myosin Heavy Chain 9 Non-muscle, Myosin Heavy Chain Non-muscle IIa, Myosin Hea

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IC IF
Myosin heavy chain type A is a critical component of the cellular contractile apparatus. It is involved in maintaining cell-cell adhesion in early mammalian embryos and ablation of this protein leads to prenatal lethality. Mutation in the human MYH9 gene lead to MYH9-related hereditary macrothrombocytopenia (HMTC) characterized by a reduced number of blood platelets.
Catalog #M9848-12
ApplicationsSuitable for use in Western Blot, Immunofluorescence and Immunocytochemistry. Other applications not tested.
Recommended DilutionsImmunofluorescence (IC): 1:50-1:100
Western Blot: 1:2000. Detects a band at ~200kD. Staining of myosin IIA (nonmuscle) is inhibited by the immunizing peptide. Additional bands may be detected in some preparations.
Optimal dilutions to be determined by the researcher.
Positive ControlMDCK or Jurkat whole cell extract (Western Blot). NRK cells (Immunofluorescence).
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.01M PBS, pH 7.4, 1% BSA, 15mM sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa1949-1960 of human non-muscle Myosin IIA, conjugated to KLH.
SpecificityRecognizes human myosin IIA. Species Crossreactivity: rat and canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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