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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin, Regulatory Light Chain of Smooth and Non-muscle, Phosphorylated pS20

Anti -Myosin, Regulatory Light Chain of Smooth and Non-muscle, Phosphorylated pS20

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule
containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000-21,000 daltons. Activation of smooth
and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle
contraction by dephosphorylating activated myosin light chain. This antibody is specific for the phosphorylated form of myosin light chain. The selected peptide sequence used to generate the
polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in
addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. pS20 is also referred to as pS19.
Catalog #M9850-02
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:5000-1:20,000
Western Blot: 1:5000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman Myosin Light Chain phosphorylated pS20, aa 12-27 KKRPQRATpSNVFAMFD, peptide corresponding to a region of the human smooth/non-muscle form of myosin regulatory light chain (KLH).
SpecificityRecognizes Human Phosphorylated pS20, Myosin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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