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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin Light Chain (MLC, A1 Catalytic, A2 Catalytic, Alkali Myosin Light Chain 1, Alkali Myosin Light Chain 3, C36E6.3, CMH10, DKFZp779C0562, MLC2, MLC-2, MLC-2v, MLC1F, MLC3F, MYL1, MYL2, Myosin Light Chain 1 Alkali Skeletal Fast, Myosin Light Chain 1 Sk

Anti -Myosin Light Chain (MLC, A1 Catalytic, A2 Catalytic, Alkali Myosin Light Chain 1,
Alkali Myosin Light Chain 3, C36E6.3, CMH10, DKFZp779C0562, MLC2, MLC-2, MLC-2v, MLC1F,
MLC3F, MYL1, MYL2, Myosin Light Chain 1 Alkali Skeletal Fast, Myosin Light Chain 1 Sk

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IF
Myosin light chain (MLC) is a subunit of the conventional myosins (e.g. myosin II). In smooth muscle and non-muscle cells conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues. Phosphorylation of serine 19 (the major phosphorylation site and the preferred site for MLCK) activates myosin motor activity under physiological conditions.
Catalog #M9850-02C
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:200. Localizes an epitope on the stress fibers of well spread human and chicken fibroblasts by indirect immunofluorescence.
Western Blot: Reacts with the 20kD light chains of SDS, heat-denatured and reduced chicken gizzard, chicken intestine, and porcine stomach myosin.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgM
Clone No8L879
HostMouse
SourceChicken
ConcentrationNot Determined
FormSupplied as liquid, 15mM sodium azide.
PurityAscites
ImmunogenChicken lens membranes. Cellular Localization: Cytoplasmic
SpecificityRecognizes chicken Myosin Light Chain at ~20kD. Does not react in Western Blot with intact myosin molecules in their native state. Species Crossreactivity: bovine, human, porcine and rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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