Anti -Myosin Light Chain, phosphorylated (Ser20) (MLC, A1 Catalytic, A2 Catalytic, Alkali
Myosin Light Chain 1, Alkali Myosin Light Chain 3, C36E6.3, CMH10, DKFZp779C0562, MLC2,
MLC-2, MLC-2v, MLC1F, MLC3F, MYL1, MYL2, Myosin Light Chain 1 Alkali Skeletal Fast,
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| Catalog # | M9850-02P | | Cellular Localization | Cytoplasmic | | Positive Control | Cardiac myocytes. | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.68mg/ml | | Form | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride pH 7.2 | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Human Myosin Light Chain phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) - KKRPQRAT(pS)NVFAMFD (aa 12-27). | | Specificity | This phospho specific polyclonal antibody is specific for phosphorylated pS20 of human myosin light chain. Reactivity with non-phosphorylated human myosin light chain is less than 1% by ELISA. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected. Species Crossreactivity: Crossreacts with Human. Expected to crossreact with all Mammals and Birds due to 100% sequence identity. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Myosin Light Chain, phosphorylated (Ser20) (MLC, A1 Catalytic, A2 Catalytic, Alkali Myosin Light Chain 1, Alkali Myosin Light Chain 3, C36E6.3, CMH10, DKFZp779C0562, MLC2, MLC-2, MLC-2v, MLC1F, MLC3F, MYL1, MYL2, Myosin Light Chain 1 Alkali Skeletal Fast, |
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