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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin Light Chain, phosphorylated (Ser20) (MLC, A1 Catalytic, A2 Catalytic, Alkali Myosin Light Chain 1, Alkali Myosin Light Chain 3, C36E6.3, CMH10, DKFZp779C0562, MLC2, MLC-2, MLC-2v, MLC1F, MLC3F, MYL1, MYL2, Myosin Light Chain 1 Alkali Skeletal Fast,

Anti -Myosin Light Chain, phosphorylated (Ser20) (MLC, A1 Catalytic, A2 Catalytic, Alkali
Myosin Light Chain 1, Alkali Myosin Light Chain 3, C36E6.3, CMH10, DKFZp779C0562, MLC2,
MLC-2, MLC-2v, MLC1F, MLC3F, MYL1, MYL2, Myosin Light Chain 1 Alkali Skeletal Fast,

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #M9850-02P
Cellular Localization Cytoplasmic
Positive Control Cardiac myocytes.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.68mg/ml
Form0.02 M Potassium Phosphate, 0.15 M Sodium Chloride pH 7.2
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman Myosin Light Chain phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) - KKRPQRAT(pS)NVFAMFD (aa 12-27).
SpecificityThis phospho specific polyclonal antibody is specific for phosphorylated pS20 of human myosin light chain. Reactivity with non-phosphorylated human myosin light chain is less than 1% by ELISA. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected. Species Crossreactivity: Crossreacts with Human. Expected to crossreact with all Mammals and Birds due to 100% sequence identity.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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