Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin Light Chain Kinase, Smooth Muscle, phosphorylated (Ser1760) (MLCK)

Anti -Myosin Light Chain Kinase, Smooth Muscle, phosphorylated (Ser1760) (MLCK)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Smooth Muscle Myosin Light Chain Kinase (MLCK) is a multifunctional regulatory protein of smooth muscle contraction (SMC) and a key element in ligand-mediated endothelial cell gap formation and vascular permeability, motility and morphology. Smooth muscle MLCK exists in at least two isoforms, short (~150kD) and long (210kD) which are identical except for an extended amino terminus with two additional putative actin-binding motifs in the long isoform.
MLCK is phosphorylated by several kinases including protein kinase A (PKA) and mediates its function by phosphorylating 20kD myosin light chain (MLC20). Phosphorylation of MLCK
inhibits the actin-activated ATPase of myosin II by reducing its affinity for actin. MLCK possesses a counter-balancing role in vascular regulation, by mediating vasoconstriction via direct
action on SMCs and vasodilation via action on endothelial cells (ECs).
Catalog #M9850-07
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Positive ControlsKCl-treated rabbit muscle extracts; COS cells over-expressing rabbit smooth muscle short MLCK, phosphorylated in vitro by PKA.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot Determined
FormSupplied as a liquid in Dulbeccos PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide derived from the region of human myosin light chain kinase (MLCK) that contains serine 1760.
SpecificityRecognizes MLCK when phosphorylated at Ser1760. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links