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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IH IC FC

Myosin light chain kinase (MLCK) is a serine/threonine kinase that belongs to CaM Kinases (Ca++/Calmodulin-dependent protein kinases) family. MLCK is involved in the regulation of myosin activity during smooth muscle contraction. At increased level of Ca2+, rapid binding of Ca2+ to Calmodulin (CaM) occurs, triggering MLCK activation. Once activated, MLCK phosphorylates myosin light chain (MLC) and leading to cross-bridges cycling and generation of the contractile force (1). MLCK has been implicated in the regulation of endothelial and vascular permeability and in signaling pathway which leads to fibroblast apoptosis.
Catalog #	M9850-07C
Applications	Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions	Western Blot: 1:1000 -1:10,000
	Immunohistochemistry: 1:100-1:250
	Immunocytochemistry: 1:100 -1:250
	Flow Cytometry: 1:50
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9E391
Host	Rabbit
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.25mM EDTA, 25mM citric acid, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Purity	Supernatant
Immunogen	A synthetic peptide corresponding to residues near the C-terminal of human MLCK protein.
Specificity	Recognizes human MLCK. Species Crossreactivity: mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.