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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin Light Chain, Phosphorylated (Ser19)

Anti -Myosin Light Chain, Phosphorylated (Ser19)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Myosin light chain (MLC) is a subunit of the conventional myosins (e.g., myosin II). In smooth muscle and non-muscle cells conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues. Phosphorylation of serine 19 (the major phosphorylation site and the preferred site for MLCK) activates myosin motor activity under physiological conditions.
Catalog #M9850-08
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000. Prior Immunoprecipitation of MLC may be necessary.
Optimal dilutions to be determined by the researcher.
Positive ControlChicken gizzard myosin II phosphorylated in vitro by PAK.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot Determined
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3 1mg/ml BSA (IgG and protease free), 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to the region of MLC that contains serine 19 (serine 20 including the initiating methionine).
SpecificityRecognizes chicken MLC when phosphorylated at Ser19. Species Sequence Homology: human, 100%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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