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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin Light Chain 2, Phosphorylated (Thr18/Ser19)

Anti -Myosin Light Chain 2, Phosphorylated (Thr18/Ser19)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC or RLC, LC20), has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). The phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fiber (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2, and Ser9 by PKC and cdc2, is also reported, which inhibits myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Lack of phosphorylation sites on cardiac muscle isoform shows morphological and functional abnormalities on transgenic mice (6).
Catalog #M9850-09A
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined.
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
PurityPurified by protein A and peptide affinity chromatography.
ImmunogenSynthetic KLH-coupled phosphopeptide corresponding to residues surrounding Thr18/Ser19 of human myosin light chain 2. MW: 18kD
SpecificityDetects endogenous levels of Thr18 and Ser19 phosphorylated myosin light chain 2 (smooth muscle). Does not crossreact with the cardiac isoform of myosin light chain 2. Species Crossreactivity:
human, mouse, rat, feline, and Xenopus.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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