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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin Light Chain 2 (MLC2, Myosin Regulatory Light Chain, MRLC, RLC, LC20)

Anti -Myosin Light Chain 2 (MLC2, Myosin Regulatory Light Chain, MRLC, RLC, LC20)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IC FC
The muscle myosins and hexomeric proteins consisting of two heavy chains and two pairs of light chains, the latter called essential (ELC) and regulatory (RLC). The light chains stabilize the long alpha helical neck of the myosin head (1). Human myosin light chain-2 (MYL2) is an important protein involved in the regulation of myosin ATPase activity in smooth muscle. In cardiac muscle, the precise role of MYL2 is not well understood; however, an increase in ventricular MYL2 is observed during myocardial hypertrophy in cardiac patients with valve stenosis (2). Familial hypertrophic cardiomyopathy (FHC) is an autosomal dominant disease caused by mutations in all of the major sarcomeric proteins, including the ventricular myosin regulatory light-chain (RLC). The Myosin RLC mutation has been associated with a rare variant of cardiac hypertrophy defined by mid-left ventricular obstruction due to papillary muscle hypertrophy. This mutation was later found to cause ventricular and septal hypertrophy (3).
Catalog #M9850-09D2
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500-1:2000
Immunocytochemistry: 1:250
Flow Cytometry: 1:20
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E392
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human Myosin RLC.
SpecificityRecognizes human Myosin RLC.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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