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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin Light Chain 2, phosphorylated (Ser19) (MLC2, Myosin Regulatory Light Chain, MRLC, RLC, LC20)

Anti -Myosin Light Chain 2, phosphorylated (Ser19) (MLC2, Myosin Regulatory Light Chain,
MRLC, RLC, LC20)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IF
Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC or RLC, LC20), has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulindependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities (6).
Catalog #M9850-09E
ApplicationsSuitable for use in Western Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunofluorescence (IC): 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No4i15
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
PurityAscites
ImmunogenSynthetic Phospho-peptide corresponding to residues surrounding
Ser19 of human Myosine Light Chain 2 (smooth muscle).
SpecificityRecognizes endogenous levels of human Myosin Light Chain 2 (smooth muscle) at ~18kD only when phosphorylated at serine 19. Does not crossreact with the cardiac isoform of Myosin Light Chain 2. Species Crossreactivity: mouse, rat, bovine, porcine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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