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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin Regulatory Light Chain (MRLC, MYL9, LC20, MLC2, MRLC1, MYRL2, Myosin Regulatory Light Polypeptide 9, Myosin Regulatory Light Chain 9, Myosin Regulatory Light Chain MRLC1, Myosin Regulatory Light Chain 2, Smooth Muscle Isoform, Myosin RLC, MLC-2C, 2

Anti -Myosin Regulatory Light Chain (MRLC, MYL9, LC20, MLC2, MRLC1, MYRL2, Myosin
Regulatory Light Polypeptide 9, Myosin Regulatory Light Chain 9, Myosin Regulatory Light
Chain MRLC1, Myosin Regulatory Light Chain 2, Smooth Muscle Isoform, Myosin RLC, MLC-2C, 2

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Myosin is a hexamer composed of two high molecular weight heavy chains and four light chain subunits, which include two alkali light chains and two MRLCs. MRLC functions to stabilize the myosin heavy chain, and may also modulate the availability of cross bridges to bind to actin. Phosphorylation of the MRLC by myosin light chain kinase (MLCK) is necessary for smooth muscle contraction via activating myosin ATPase.
Catalog #M9850-09F
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:5000-1:20,000
Western Blot: 1:500-1:2000. Detects a band of ~22 and 24kD (phosphorylated and non-phosphorylated forms) with rat aorta cell lysates.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.01% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa12-27 of human Myosin Regulatory Light Chain.
SpecificityRecognizes human Myosin Regulatory Light Chain. Does not detect the sarcomeric/cardiac myosin regulatory light chain isoform. Species Sequence Homology: mouse, birds and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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