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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin Regulatory Light Chain (MRLC, MYL9, LC20, MLC2, MRLC1, MYRL2, Myosin Regulatory Light Polypeptide 9, Myosin Regulatory Light Chain 9, Myosin Regulatory Light Chain MRLC1, Myosin Regulatory Light Chain 2, Smooth Muscle Isoform, Myosin RLC, MLC-2C, 2

Anti -Myosin Regulatory Light Chain (MRLC, MYL9, LC20, MLC2, MRLC1, MYRL2, Myosin
Regulatory Light Polypeptide 9, Myosin Regulatory Light Chain 9, Myosin Regulatory Light
Chain MRLC1, Myosin Regulatory Light Chain 2, Smooth Muscle Isoform, Myosin RLC, MLC-2C, 2

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Myosin is a hexamer composed of two high molecular weight heavy chains and four light chain subunits, which include two alkali light chains and two MRLCs. MRLC functions to stabilize the myosin heavy chain, and may also modulate the availability of cross bridges to bind to actin. Phosphorylation of the MRLC by myosin light chain kinase (MLCK) is necessary for smooth muscle contraction via activating myosin ATPase.
Catalog #M9850-09G
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:5,000-1:20,000
Western Blot: 1:500-1:2,000
Immunohistochemistry (paraffin): 2.5ug/ml
Requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.
Positive Control Smooth muscle
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide corresponding to aa12-27 of human Myosin Regulatory Light Chain.
SpecificityRecognizes human myosin regulatory light chain (MRLC), also known as MYL9 and LC20. Species crossreactivity: birds, mammals, mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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