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You are here:Home » Molecular Biology » MB-Myosin » Myosin Light Chain 2, Non-muscle, Recombinant, Human (MLC2, Myosin Regulatory Light Chain, MRLC, RLC, LC20)

Myosin Light Chain 2, Non-muscle, Recombinant, Human (MLC2, Myosin Regulatory Light Chain,
MRLC, RLC, LC20)

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Specifications

Non muscle Myosins are required for cytokinesis at the end of cell division, when a contractile ring near the plasmalemma divides the cytoplasm of the daughter cells. They are involved in cytoplasmic streaming movements in tissues, and especially in activation of motile cells such as fibroblasts and macrophages. Activation of non-muscle myosin-II is achieved by phosphorylation of Ser33 in the motif KKRPQRATSN by a dedicated, Ca+2 Calmodulin regulated light chain kinase (MLCK).
Catalog #M9850-09N
ActivityAssayed for phosphorylation by MLCK.
ApplicationsSuitable for use in ELISA, Western Blot and Inhibition Assays. Other applications have not been tested.
Amino Acid SequenceMSSKKAKTKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID KEDLHDMLAS LGKNPTDAYL DAMMNEAPGP INFTMFLTMF GEKLNGTDPE DVIRNAFACF DEEATGTIQE DYLRELLTTM GDRFTDEEVD ELYREAPIDK KGNFNYIEFT RILKHGAKDK DD
Storage and StabilityAliquot to avoid repeated freezing and thawing and freeze at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
SourceE. coli
Purity~90% by SDS-PAGE
Concentration~0.5mg/ml
FormSupplied as a liquid in 20mM HEPES-KOH, pH 7.0, 50mM sodium chloride, 1mM EDTA and 1mM DTT.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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