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You are here:Home » Antibodies » Antibodies-Myosin » Anti -Myosin Light Chain Kinase 3, NT (MYLK3, MLCK2, MLCK, caMLCK, Cardiac-MyBP-C-associated Ca/CaM Kinase, MGC126319, MGC126320, Putative Myosin Light Chain Kinase 3)

Anti -Myosin Light Chain Kinase 3, NT (MYLK3, MLCK2, MLCK, caMLCK,
Cardiac-MyBP-C-associated Ca/CaM Kinase, MGC126319, MGC126320, Putative Myosin Light Chain
Kinase 3)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B IH
MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.
Catalog #M9850-09W
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Immunohistochemistry (FFPE): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs Reported
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by ammonium sulfate precipitation.
ImmunogenKLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 40-69 in the N-terminal region of human MLCK3., UniProt Accession # Q32MK0
SpecificityRecognizes human MLCK3. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=2.7.11.18


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