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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin, Cardiac, Heavy Chain (Alpha MHC, ASD3, CMD1S, CMH1, MGC138376, MGC138378, MPD1, MYH6, MYH7, MYHC, MyHC-alpha, MYHCA, MyHC-beta, MYHCB, Myosin Heavy Chain Cardiac Muscle alpha Isoform, Myosin Heavy Chain Cardiac Muscle beta Isoform, Myosin Heavy Po

Anti -Myosin, Cardiac, Heavy Chain (Alpha MHC, ASD3, CMD1S, CMH1, MGC138376, MGC138378,
MPD1, MYH6, MYH7, MYHC, MyHC-alpha, MYHCA, MyHC-beta, MYHCB, Myosin Heavy Chain Cardiac
Muscle alpha Isoform, Myosin Heavy Chain Cardiac Muscle beta Isoform, Myosin Heavy Po

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IH IC IF
Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Cardiac MHC exists as two isoforms in humans, alpha-cardiac MHC and beta-cardiac MHC. These two isoforms are expressed in different amounts in the human heart. During normal physiology, beta-cardiac MHC is the predominant form, with the alpha-isoform contributing around only 7% of the total MHC. Mutations of the MHC genes are associated with several different dilated and hypertrophic cardiomyopathies.
Catalog #M9850-11C
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:100-1:2000
Immunohistochemistry: 1:10-1:2000
Immunohistochemistry (Frozen): 10ug/ml tissue treatment may reduce or destroy the myosin binding sites
Immunohistochemistry(Paraffin): 1:400
Western Blot: 1:100-1:2000. Detects a band at ~223kD
Immunocytochemistry (IF): 1:10-1:2000
Indirect Immunofluorescence (Frozen): Binds to A-band of thick filament of cardiac and skeletal muscle myosin and indirect immunoperoxidase staining of Carnoy fixed tissues.
Optimal dilutions to be determined by the researcher.
Affinity ConstantsHuman ventricular myosin heavy chains: 3.33 x10e8/M Human atrial myosin heavy chains: 1.48 x10e8/M
Human skeletal muscle myosin: 1.06 x10e8/M
Canine cardiac myosin heavy chains: 2.06 x10e8/M
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No8L880
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No preservatives added.
PurityPurified
ImmunogenPurified full length native human Myosin Cardiac, Heavy Chain.
SpecificityRecognizes human myosin alpha and beta heavy chains. Reacts with heavy chains of human myosin isolated from
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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