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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin, Heavy Chain (Smooth Muscle and Non-Muscle)

Anti -Myosin, Heavy Chain (Smooth Muscle and Non-Muscle)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified B IF
Catalog #M9850-12B
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:25-1:100; Cells were fixed at 37ºC for 5-10 minutes in freshly prepared formaldehyde at a concentration of 2-4%, buffered with a balanced salt solution.
Western Blot: Major bands were observed at 190-200kD. Fragments of heavy chain myosin may also be seen at 170kD, 130kD and 110kD.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceBovine
ConcentrationNot Determined
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified by ammonium sulfate precipitation.
ImmunogenBovine uterine myosin.
SpecificityRecognizes bovine heavy chain myosin from smooth muscle or non-muscle sources. Does not cross react with Myosin Skeletal and Cardiac, Actin, Tropomyosin or Tubulin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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