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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin, Smooth Muscle, Heavy Chain 2 (MHC-2)

Anti -Myosin, Smooth Muscle, Heavy Chain 2 (MHC-2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified B IH IF
The heavy chain of myosin II found in vertebrate smooth muscle is a non-striated muscle present in the walls of hollow organs, the vasculature, the respiratory and gastrointestinal tracts, uterus, bladder and eye.
Catalog #M9850-12C
The structure of myosin II is fundamentally the same in all muscle types and consists of two heavy chains, each bound by 2 distinct, essential and regulatory light chains. The globular motor domain located at the N-terminal domain of the heavy chains, catalyzes ATP hydrolysis and interacts with actin, whilst the heptad repeat sequences of the heavy chain tail domains promote dimerization, forming an alpha-helical coiled coil at the C-terminal domain.
ApplicationsSuitable for use in Immunofluorescence, Immunohistochemistry (frozen), and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:150
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceBovine
ConcentrationNot determined
FormSupplied as a liquid in 20mM TRIS, pH 7.5, 150mM sodium chloride, 0.006% sodium azide.
PurityPurified by Ion Exchange chromatography.
ImmunogenBovine tracheal smooth muscle myosin.
SpecificityRecognizes the heavy chain of myosin II found in vertebrate smooth muscle. Does not crossreact with myosin of skeletal or non-muscle origin. Species Crossreactivity: vertebrates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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