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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin, Skeletal Muscle, heavy chain

Anti -Myosin, Skeletal Muscle, heavy chain

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant IH
Myosin is a 480kD protein known to interact with actin in muscle and in nonmuscle cells. It contains two identical heavy chains (200kD each) and four light chains (1526kD). Myosin molecules consist of two major regions: tails (rod) and heads; they aggregate into filaments through the tail region and interact with actin and with ATP through the head region. Multiple forms of myosin heavy chains exist for each muscle type. Skeletal, cardiac, smooth and non-muscle isomyosin forms exist in different types of skeletal muscle, depending on the physiological function of the muscle. These are designated at type I (slow-twitch) and type II (fast-twitch).
Catalog #M9850-15
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 1:250-1:500 for 30 min at RT. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Optimal dilutions to be determined by the researcher.
HybridomaHybridization of NS1 myeloma cells with splenocytes from Balb/c mice.
Positive ControlSkeletal muscle
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2N1
HostMouse
SourceRabbit
ConcentrationNot determined
FormSupplied as a liquid with 0.09% sodium azide, before the addition of glycerol to 40%.
PurityTissue culture supernatant
ImmunogenRabbit muscle myosin. Cellular Localization
SpecificityRecognizes rabbit skeletal muscle myosin heavy chain. Does not crossreact with cardiac or smooth muscle myosin. May be used for detecting cross-striated muscle differentiation in tumors. Species Crossreactivity: Human, bovine, mouse, rat, chicken and guinea pig.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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