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You are here:Home » Antibodies » Antibodies-Myosin » Anti -Myosin, Skeletal Muscle, heavy chain

Anti -Myosin, Skeletal Muscle, heavy chain


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Clone Host Grade Applications
Monoclonal Mouse Ascites IH
Myosin is a highly conserved, ubiquitous protein found in all eukaryotic cells, where it provides the motor function for diverse movements such as cytokinesis, phagocytosis, and muscle contraction. All myosins contain an amino-terminal motor/head domain and a carboxy-terminal tail domain. Based on the properties of the head domain, myosin is divided into seven distinct classes. Class II myosin, one of the widely studied myosin consists of the conventional two-headed myosins that form filaments and are composed of two myosin heavy chain (MYH) subunits and four myosin light chain subunits. The MYH subunit contains the ATPase activity providing energy that is the driving force for contractile processes. Numerous MYH isoforms exist in vertebrates to carry out this function. In humans, the skeletal myosin heavy chain (MYH) gene is clustered on a single chromosome (17p).
Catalog #M9850-15H
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (paraffin): 1:25-1:50
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.
Positive ControlSkeletal muscle
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No11E287
ConcentrationNot determined.
FormSupplied as a liquid, 0.05% sodium azide.
ImmunogenRabbit muscle myosin. Cellular Localization: Cytoplasmic.
SpecificityRecognizes heavy chain of skeletal myosin. It does not stain cardiac or smooth muscle myosin. This antibody stains the fast twitch (type II) isomyosin molecule. Species Crossreactivity: bovine, rabbit, chicken, guinea pig, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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