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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin, Slow Skeletal (Slow Myosin Heavy Chain 1, SM1)

Anti -Myosin, Slow Skeletal (Slow Myosin Heavy Chain 1, SM1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IH IF
Catalog #M9850-25H
ApplicationsSuitable for use in RIA (solid phase), Immunofluorescence, ELISA, Western Blot and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.
Recommended DilutionImmunohistochemistry (paraffin): 1:4000. Requires enzymatic antigen retrieval; proteolytic digestion greatly enhances staining in formalin-fixed, paraffin-embedded tissue sections.
Optimal dilutions to be determined by the researcher.
Cellular Localization Cytoplasmic
Positive Control rabbit tounge
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Numbern/a
Clone TypeMonoclonal
IsotypeIgG1
Clone No8L883
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as liquid (ascites) containing 15mM sodium azide.
PurityAscites
ImmunogenMyosin purified from myofibrils isolated from human skeletal muscle
SpecificityRecognizes an epitope located on the heavy meromyosin portion of human adult skeletal muscle slow myosin. In rat, the product is highly specific for the slow myosin heavy chain of adult skeletal muscle. It does not react with the fast myosin heavy chain. It does not crossreact with embryonic or neonatal rat skeletal myosins, nor with cat fetal/embryonic skeletal myosin. The product shows no reactivity with human or rat adult smooth muscle or with non-muscle tissues. Species Crossreactivity: Crossreacts with Human, Mouse, Rat, bovine, Cat, Chicken, canine, Goat, Guinea pig, Hamster, Pig, Rabbit and Sheep.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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