You are here:Home
»
Antibodies
»
Abs to Myosin
»
Anti -MYPT1 (Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, MYPT1, Protein phosphatase 1 regulatory subunit 12A, Protein phosphatase myosin-binding subunit)
Anti -MYPT1 (Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1,
MYPT1, Protein phosphatase 1 regulatory subunit 12A, Protein phosphatase myosin-binding
subunit)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Protein phosphatase 1 (PP1) is a ubiquitous eukaryotic protein serine/threonine phosphatase involved in the regulation of various cell functions. Substrate specificity is determined by the binding of a regulatory subunit to the PP1 catalytic subunit (PP1c). It is estimated that over fifty different regulatory subunits exist (1). The myosin phosphatase holoenzyme is composed of three subunits: the PP1 catalytic subunit (PP1c), a targeting/regu- latory subunit (MYPT/myosin-binding subunit of myosin phosphatase) and a 20 kD subunit of unknown function (M20). MYPT binding to PP1c delta alters the conforma- tion of the catalytic cleft and increases enzyme activity and specificity (2). Two MYPT isoforms that are 61% identical have been described. MYPT1 is widely expressed while MYPT2 expression appears to be exclusive to heart and brain (3). Related family members also include MBS85, MYPT3 and TIMAP (4). | | | Catalog # | M9925-01C | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Form | Supplied as a liquid in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol. | | Purity | Protein A and peptide affinity chromatography. | | Immunogen | Synthetic peptide (KLH-coupled) correspondent to amino-terminal residues of human MYPT1. | | Specificity | Detects endogenous levels of total MYPT1 protein. The antibody is unlikely to cross-react with MYPT2 and does not cross-react with other family members. Species sequence homology: Chicken, Bovine and Zebra fish. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
