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You are here:Home » Antibodies » Abs to Myosin » Anti -MYPT1 (Myosin Phosphatase 1, M130, MBS, MGC133042, Myosin Phosphatase Target Subunit 1, Myosin Phosphatase Targeting Subunit 1, Protein Phosphatase 1 Regulatory Inhibitor Subunit 12A, Protein Phosphatase 1 Regulatory Subunit 12A, PPP1R12A, Protein Phospha

Anti -MYPT1 (Myosin Phosphatase 1, M130, MBS, MGC133042, Myosin Phosphatase Target Subunit
1, Myosin Phosphatase Targeting Subunit 1, Protein Phosphatase 1 Regulatory Inhibitor
Subunit 12A, Protein Phosphatase 1 Regulatory Subunit 12A, PPP1R12A, Protein Phospha

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.
Catalog #M9925-01D
ApplicationsSuitable for use in Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionsImmunoprecipitation: 2-5ug/mg lysate
Western Blot: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlHeLa whole cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in Tris-citrate/phosphate buffer, pH 7.5, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
SpecificityRecognizes human MYPT1. The epitope recognized maps to a region between aa400-450 of human MYPT1 (NP_002471.1).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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