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You are here:Home » Antibodies » Abs to Myosin » Anti -MYPT1, phosphorylated (Thr850) (Myosin Phosphatase Target Subunit 1, Myosin Phosphatase-targeting Subunit 1, MBS, MGC133042, Protein Phosphatase 1 Regulatory Subunit 12A, PPP1R12A, Protein Phosphatase Myosin-binding Subunit)

Anti -MYPT1, phosphorylated (Thr850) (Myosin Phosphatase Target Subunit 1, Myosin
Phosphatase-targeting Subunit 1, MBS, MGC133042, Protein Phosphatase 1 Regulatory Subunit
12A, PPP1R12A, Protein Phosphatase Myosin-binding Subunit)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B
Phosphorylation of myosin is crucial to smooth muscle contraction. The degree of myosin phosphorylation is dependent in part by the activity of myosin phosphatase. Recent findings suggest that this enzyme can be regulated, which has lead to interest in characterizing this phosphatase. Myosin phosphatase seems to be comprised of three subunits (catalytic subunit of type 1 phosphatase-delta (PP1) and two non-catalytic subunits, large and small (M20)). Studies suggest that the large subunit is a targeting subunit and is termed myosin phosphatase target subunit (MYPT). MYPT has been shown to be a substrate for the Rho-associated kinase (ROK). ROK mediates an agonist-induced increase in myosin phosphorylation and force by inhibiting myosin light chain phosphatase activity through phosphorylation of MYPT.
Catalog #M9925-09B
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000 detected MYPT1 in RIPA lysates from HEK293 cells phosphorylated by preincubation with ROKa/ROCK-II, active.
Optimal dilutions to be determined by the researcher.
Positive ControlHEK293 cell lysate phosphorylated by ROK alpha/ROCKII.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No10F650
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid, 0.05% sodium azide.
PuritySupernatant
ImmunogenSynthetic peptide containing the sequence ...RS[pT]GV…, in which [pT] corresponds to phosphorylated threonine at aa residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1), conjugated to KLH. Corresponds to Thr853 in human MYPT1.
SpecificityRecognizes human phospho-MYPT1 (Thr850) ay ~130kD. A non-specific protein was also detected at ~55-60kD.
Species crossreactivityChicken. Species Sequence Homology: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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